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Janice VanCleave's Science for Fun
In the Lab
Biology for Ages 13+
Phototropism: Plant Movement Due to Light
Don't missA+ Projects in Biologyfor this experiment and others.

Purpose
To determine the response of oat seedlings to light.

    Materials
  • flowerpot
  • tap water
  • 1 tablespoon (15 ml) of oat seeds
  • potting soil
Procedure
1. Fill the flowerpot with potting soil to within 2 inches (5 cm) from the top.
2. Sprinkle the oat seeds over the surface of the soil.
3. Cover the seeds with about 1 inch (2.5 cm) of soil.
4. Moisten the soil with water and keep it moist, but not dripping wet.
5. Place the pot on a table near a window.
6. Allow the pot to remain undisturbed for 14 days.
7. Make daily observations and record the growth of the oat seeds above the surface of the soil.

Results
The first signs of growth generally appear in four to six days. Straight, closed tube- like structures break through the soil and grow toward the light. After several more days, leaves break through the ends of these tubes. The tubes and leaves bend toward the light.

Why?
As the seeds germinate, each primary leaf is protected by a hollow, cylindrical structure, the coleoptile, which surrounds it. After the coleoptile has grown above the surface of the soil, it stops growing and the primary leaf breaks through. The coloeptile shoot and the leaf bend toward the light as a result of the buildup of the growth hormone auxin. The auxin migration theory for this phototropism (growth toward the light) state that light-sensitive auxin moves from the light side of the dark side of unevenly lighted growing tips. The cells on the shaded side contain a higher concentration of auxin which promotes cell elongation. Thus, the cells on the shaded side are longer than on the lighted side. As a result, the plant bends toward the light.

 

For ideas on how to develop this experiment into a science fair project, see page 18-88 in "Janice VanCleave's A+ Projects in Biology" (Wiley, 1993).


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